Guanidine extraction of streptococcal M protein
نویسندگان
چکیده
منابع مشابه
Superantigenicity of streptococcal M protein
M proteins that define the serotypes of group A streptococci are powerful blastogens for human T lymphocytes. The mechanism by which they activate T cells was investigated and compared with the conventional T cell mitogen phytohemagglutinin, and the known superantigen staphylococcal enterotoxin B. Although major histocompatibility complex (MHC) class II molecules are required for presentation, ...
متن کاملStreptococcal M Protein Extracted by Nonionic Detergent
Streptococcal M proteins are antiphagocytic molecules varying immunologically from type to type while maintaining an identical biological effect. The virulence of the group A streptococcus is directly related to the presence of the M antigens on the cell surface and resistance to infection by these organisms is dependent on the presence of opsonic antibodies directed towards the M molecules. Va...
متن کاملFibrinogen Precipitation by Streptococcal M Protein
Evidence confirming the identity of fibrinogen-precipitating factor and streptococcal M protein is provided by the demonstration of bactericidal, mouse protective, and long chain-producing antibodies in the sera of rabbits immunized with washed M-fibrinogen precipitates. Two precipitin lines were observed in immunoelectrophoresis of human plasma vs. rabbit anti-M-fibrinogen antiserum; no precip...
متن کاملStreptococcal M6 protein expressed in Escherichia coli. Localization, purification, and comparison with streptococcal-derived M protein
Type 6 streptococcal M protein produced by E. coli bearing plasmid pJRS42.13 (ColiM6) accumulates in the periplasmic space of this new host. No immunoreactive M protein was found either on the surface of the organism or in the culture medium. The ColiM6 protein was purified from the periplasm and the final preparation consisted of three protein bands of apparent molecular weight 55,000, 57,000,...
متن کاملSequence of myosin-crossreactive epitopes of streptococcal M protein
Group A streptococcal M proteins contain epitopes that crossreact with sarcolemmal membrane proteins of human myocardium and myosin. In the present study, synthetic peptide copies spanning the entire 197-residue pepsin extracted fragment of type 5 M protein were used to localize the myosin-crossreactive epitopes of the molecule. Peptide 84-116 inhibited by 75% the binding of myosin-crossreactiv...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1975
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.12.3.679-686.1975